The Science of Error: Mesmerism and American Fiction, 1784-1890

Antebellum mesmerism posed a challenge to the prerogatives of self-mastering reason from within the scientific tradition itself. Though mesmerism is now most familiar as the sensational stage-practice and quack cure that drew criticism from Hawthorne and Henry James, its sympathizers considered it a theory of sensory error. Mesmerists claimed that the trance replicated the physiological effects of deception, allowing them to study swindling in laboratory conditions. Concluding that sensory error was ineradicable, they refuted Lockean pedagogy\u27s claims to reform the errant senses. One could at best manage delusion, through the self-doubt that liberalism had enjoined only its marginalized types—credulous women, laborers, racial inferiors—to practice. Mesmerism transvalued these figures, praising their powers of self-suspicion and condemning the ridiculous confidence of reason. Tracing the American mesmeric tradition from the science\u27s first appearance there as a falsehood, in 1784; through its limited practice in the 1790s; to its extensive popularity from the 1840s to the end of the century, I find in its performances an alternate sensory public capable of including among its knowing subjects hysterics, renegades, and castaways. Rather than thinking of American publics as being formed through agreement on the procedures of reason, then, my project proposes that we see them as forming around the procedures of sensation that mesmerism discloses. Through readings of The Coquette (1797), Edgar Huntly (1799), Moby-Dick (1851), The Blithedale Romance (1853), and other works of fiction, I argue that this tradition constitutes a resource for the novel in holding open the gates of the public sphere to a pluralistic range of knowledge-producers. Forming oxymoronic crosses between good liberals and strange, errant, but insightful mesmeric knowers, American fiction creates stereoscopic images of impossible subjects

‘Go Fish’: Conceptualising the challenges of engaging national web archives for digital research

Our work considers the sociotechnical and organisational constraints of web archiving in order to understand how these factors and contingencies influence research engagement with national web collections. In this article, we compare and contrast our experiences of undertaking web archival research at two national web archives: the UK Web Archive located at the British Library and the Netarchive at the Royal Danish Library. Based on personal interactions with the collections, interviews with library staff and observations of web archiving activities, we invoke three conceptual devices (orientating, auditing and constructing) to describe common research practices and associated challenges in the context of each national web archive. Through this framework we centre the early stages of the research process that are often only given cursory attention in methodological descriptions of web archival research, to discuss the epistemological entanglements of researcher practices, instruments, tools and methods that create the conditions of possibility for new knowledge and scholarship in this space. In this analysis, we highlight the significant time and energy required on the part of researchers to begin using national web archives, as well as the value of engaging with the curatorial infrastructure that enables web archiving in practice. Focusing an analysis on these research infrastructures facilitates a discussion of how these web archival interfaces both enable and foreclose on particular forms of researcher engagement with the past Web and in turn contributes to critical ongoing debates surrounding the opportunities and constraints of digital sources, methodologies and claims within the Digital Humanities

Genomic data reveals strong differentiation and reduced genetic diversity in island golden eagle populations

Understanding population structure and the extent and distribution of genetic diversity are recognised as central issues in endangered species research, with broad implications for effective conservation management. Advances in whole genome sequencing (WGS) techniques provide greater resolution of genome-wide genetic diversity and inbreeding. Subspecies of golden eagles (Aquila chrysaetos) in Scotland (A. c. chrysaetos) and Japan (A. c. japonica) are endangered; it is therefore important to understand genetic diversity and inbreeding of these small island populations to increase the chances of conservation success. We investigated this using WGS data from golden eagles in Scotland, continental Europe, Japan, and the USA. Following determination of population genetic structure, analysis of heterozygosity and nucleotide diversity revealed reduced levels of genetic diversity together with runs of homozygosity (ROH), suggesting evidence of inbreeding due to recent shared parental ancestry in the island populations. These results highlight the need to consider genetic reinforcement of small isolated golden eagle populations from neighbouring outbred populations, alongside existing efforts to boost population size through within-island conservation translocations and captive breeding programmes

Update on caseous lymphadenitis in sheep

Background: Caseous lymphadenitis (CLA), caused by the gram-positive bacteria Corynebacterium pseudotuberculosis, has been present in Great Britain since the 1980s and is now considered endemic. CLA is considered to be an iceberg disease; that is, it is a production-limiting disease, characterised by slow insidious onset, that has production-limiting effects in a larger proportion of the flock than is exhibiting clinical signs at any given point in time. Aim of the article: The disease has been previously reviewed in In Practice (Baird 2003). In this article we consider updates in our understanding of the pathology, risk factors for flocks and the challenges of initiating control where the cost of the disease is still relatively unquantified

Developmental validation of Oxford Nanopore Technology MinION sequence data and the NGSpeciesID bioinformatic pipeline for forensic genetic species identification

Species identification of non-human biological evidence through DNA nucleotide sequencing is routinely used for forensic genetic analysis to support law enforcement. The gold standard for forensic genetics is conventional Sanger sequencing; however, this is gradually being replaced by high-throughput sequencing (HTS) approaches which can generate millions of individual reads in a single experiment. HTS sequencing, which now dominates molecular biology research, has already been demonstrated for use in a number of forensic genetic analysis applications, including species identification. However, the generation of HTS data to date requires expensive equipment and is cost-effective only when large numbers of samples are analysed simultaneously. The Oxford Nanopore Technologies (ONT) MinION™ is an affordable and small footprint DNA sequencing device with the potential to quickly deliver reliable and cost effective data. However, there has been no formal validation of forensic species identification using high-throughput (deep read) sequence data from the MinION making it currently impractical for many wildlife forensic end-users. Here, we present a MinION deep read sequence data validation study for species identification. First, we tested whether the clustering-based bioinformatics pipeline NGSpeciesID can be used to generate an accurate consensus sequence for species identification. Second, we systematically evaluated the read variation distribution around the generated consensus sequences to understand what confidence we have in the accuracy of the resulting consensus sequence and to determine how to interpret individual sample results. Finally, we investigated the impact of differences between the MinION consensus and Sanger control sequences on correct species identification to understand the ability and accuracy of the MinION consensus sequence to differentiate the true species from the next most similar species. This validation study establishes that ONT MinION sequence data used in conjunction with the NGSpeciesID pipeline can produce consensus DNA sequences of sufficient accuracy for forensic genetic species identification

Thermal annealing behaviour and gel to crystal transition of a low molecular weight hydrogelator

The thermal annealing behaviour of an electrolyte-triggered calixarene hydrogelator is found to depend strongly on the specific metal chloride used. While the lithium chloride gel showed typical gel-sol transitions as a function of temperature, the magnesium chloride gel was found to repeatedly strengthen with heat-cool cycles. Structural investigations using small-angle neutron scattering, and scanning probe microscopy, suggest that the annealing behaviour is associated with a change in morphology of the fibrous structures supporting the gel. On prolonged standing at room temperature, the magnesium chloride gel underwent a gel-crystal transition, with the collapsing gel accompanied by the deposition of crystals of a magnesium complex of the proline-functionalised calix[4]arene gelator

Comparative population genomics of manta rays has global implications for management

Understanding population connectivity and genetic diversity is of fundamental importance to conservation. However, in globally threatened marine megafauna, challenges remain due to their elusive nature and wide-ranging distributions. As overexploitation continues to threaten biodiversity across the globe, such knowledge gaps compromise both the suitability and effectiveness of management actions. Here, we use a comparative framework to investigate genetic differentiation and diversity of manta rays, one of the most iconic yet vulnerable groups of elasmobranchs on the planet. Despite their recent divergence, we show how oceanic manta rays (Mobula birostris) display significantly higher heterozygosity than reef manta rays (Mobula alfredi) and that M. birostris populations display higher connectivity worldwide. Through inferring modes of colonisation, we reveal how both contemporary and historical forces have likely influenced these patterns, with important implications for population management. Our findings highlight the potential for fisheries to disrupt population dynamics at both local and global scales and therefore have direct relevance for international conservation of marine species